FastQCFastQC Report
Thu 16 Oct 2025
HPAP-155_exocrine_5000149_NSS0011_s_2_CAATGCGA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-155_exocrine_5000149_NSS0011_s_2_CAATGCGA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22260093
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG825050.37064085940701147No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA423240.19013397652920858No Hit
CTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCGG388750.17463988133382913No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA358190.16091127741469902No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA355990.15992296168753653No Hit
GTCGATTTGGCGAGGGCGCTCCCGACGACGCACCGGGAGGAGGCCCTTCC342300.15377294245805712No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA336520.15117636750214836No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG334150.15011168192334146No Hit
GCGAAATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTG293670.13192667254355137No Hit
CGGTGATGTAGAGGGTGATGGTAGATGTGGCGGGTTTTAGGGGCTCTTTG289210.12992308702394012No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT282860.12707044844781196No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC274010.12309472381809006No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC260950.11722772227411628No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT250200.11239845224366313No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC249590.11212441924658627No Hit
GTTTATTATAATAATGTTTGTATATTCGGCTATGAAGAATAGGGCGAAGG233590.10493666850358621No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG227080.10201215242002808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)