FastQCFastQC Report
Thu 16 Oct 2025
HPAP-156_alpha_5000150_NSS0022_s_2_CGAGAGAA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-156_alpha_5000150_NSS0022_s_2_CGAGAGAA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31798268
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT773650.24329941492410842No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG522890.1644397738895716No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA457540.14388834008191892No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG410080.12896299886522122No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC380330.11960714338277796No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC362630.11404080247389575No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC359060.11291809981600257No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG356820.11221365893261859No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG328640.10335154103361857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)