FastQCFastQC Report
Thu 16 Oct 2025
HPAP-156_exocrine_5000152_NSS0022_s_2_ATAACGCC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-156_exocrine_5000152_NSS0022_s_2_ATAACGCC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70143851
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2312290.3296497079979256No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2142980.30551216813003323No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG1839010.2621769369349282No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1797060.2561963699426768No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1532550.21848672095291718No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG1514220.2158735196902719No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC1363770.19442474009589236No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1326150.1890614759660116No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC1280810.18259761643255087No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG1169550.16673592671722573No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT1107640.15790977886286853No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC1041560.14848913841357242No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG1034340.1474598250957165No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC1018790.14524295222969721No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT976970.1392809185797341No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT968750.13810904109043No Hit
ATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCA919600.13110201206375166No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG900370.12836050304680305No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG890020.12688496387231435No Hit
GGAGCGATAGGCATTGGTGCCTTCTGGGCAGCTGATCCGGGCCTGGGGCA889600.12682508692030608No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT877830.12514710662236095No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG848230.12092720714749466No Hit
GGGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAG846810.12072476602403824No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC837880.11945166797300592No Hit
CTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCAC832020.11861624192831956No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG829880.11831115460142044No Hit
GTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAA819300.11680282566749864No Hit
GCGATAGGCATTGGTGCCTTCTGGGCAGCTGATCCGGGCCTGGGGCAACT806190.11493380937981292No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT788430.11240186969489313No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC782040.11149088463933923No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT776340.11067826886208458No Hit
GGTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGT773840.11032185843346412No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC769860.10975445303110032No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG754750.10760030840051825No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC737770.10517956876932805No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG732290.104398317109792No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG730900.10420015291147901No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT724600.10330199863135543No Hit
CGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAG724130.10323499347077479No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC704810.10048065367839584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)