FastQCFastQC Report
Thu 16 Oct 2025
HPAP-157_exocrine_5000155_NSS0011_s_1_GTAACCGA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-157_exocrine_5000155_NSS0011_s_1_GTAACCGA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21976578
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG961930.43770690778154814No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG591750.26926394091018174No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG459220.20895882880401123No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT436340.19854774478538015No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC388190.17663805529687107No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC370500.16858857643806055No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC307220.1397942846242941No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC295430.13442948215140685No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA288340.13120332018933975No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG279490.12717630561045493No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC262810.11958640694652278No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG258110.1174477664357026No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC247330.11254254415769371No Hit
GCGATAGGCATTGGTGCCTTCTGGGCAGCTGATCCGGGCCTGGGGCAACT224410.10211325894322583No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG222920.10143526439830622No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG220910.10052065430750867No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG219810.10002012142199755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)