FastQCFastQC Report
Thu 16 Oct 2025
HPAP-158_exocrine_5000158_NSS0011_s_1_AACCAGAG_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-158_exocrine_5000158_NSS0011_s_1_AACCAGAG_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27119889
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG764810.2820107412681519No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC665690.24546191911036216No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT594380.21916756370204907No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT587010.21645000095686234No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG563440.20775896243528136No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA529240.19514829135178247No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG517860.19095210898540182No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC512590.18900888569271063No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG474310.17489378367293465No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC462730.17062385469203062No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC455140.16782517067086816No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC440500.16242691848775637No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG435540.1605980024475764No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG410380.1513206783405345No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA396710.14628009723786112No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG396410.14616947731607605No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT375620.13850351673637012No Hit
CAGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCT343440.12663768645955742No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC336760.12417454953447633No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC334520.12334858745181441No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG327500.1207600812820436No Hit
GCGATAGGCATTGGTGCCTTCTGGGCAGCTGATCCGGGCCTGGGGCAACT315420.11630578576483112No Hit
ATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCA310390.11445105840956797No Hit
CTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCAC309000.11393851943863045No Hit
GGAGCGATAGGCATTGGTGCCTTCTGGGCAGCTGATCCGGGCCTGGGGCA308530.11376521489450048No Hit
GTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAA304150.11215016403643835No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT295890.10910442885662254No Hit
CGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATTGTCCAGAGTCCGG289230.10664866659299378No Hit
CTCGGTGCCAGCAGCTGGAGGGGCAGTGGGCAGAGAGATGGCGGACACGC283330.10447314146455394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)