FastQCFastQC Report
Thu 16 Oct 2025
HPAP-159_alpha_5000159_NSS0022_s_2_CCTTCCAT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-159_alpha_5000159_NSS0022_s_2_CCTTCCAT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86259531
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3063600.35516075319259505No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC2540780.29455063927950176No Hit
CTCAGAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCA1738380.2015290345132992No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG1362450.15794776347671077No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG1287480.1492565499805465No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT1260350.14611139028799033No Hit
CTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATG1140910.13226480445389854No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1096960.12716971530948853No Hit
GTGGAATAGGAGTAGGGGCTCAGCAGGGCGGCAATGGTGTAGCGGCGGGG1073410.12443958221845654No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT1061060.1230078563724164No Hit
CGACAGCCGTGGTGGAATAGGAGTAGGGGCTCAGCAGGGCGGCAATGGTG1045360.12118776764506174No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG1023260.11862573192056886No Hit
GGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCAG937220.10865118197779212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)