FastQCFastQC Report
Thu 16 Oct 2025
HPAP-159_beta_5000160_NSS0022_s_2_GTCAGTCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-159_beta_5000160_NSS0022_s_2_GTCAGTCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28909993
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG1697320.5871049501810671No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1198310.414496814302238No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT1147060.39676938005484813No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT1131910.3915289775407417No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG978050.3383086256714071No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC910260.31485998630300605No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA861530.2980042229688537No Hit
AGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAG817680.28283645727620893No Hit
TGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTT756540.26168806059551797No Hit
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT733120.2535870555209059No Hit
AGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGC634160.21935667711853132No Hit
AGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAG591060.2044483372929215No Hit
CGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAG547700.1894500631667396No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA504850.17462819863014148No Hit
AGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGAC488960.16913182926056053No Hit
CTGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCT430170.14879630029657911No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC424980.1470010732966971No Hit
GAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCA413790.1431304393605353No Hit
GGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGG405700.14033209900811808No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT404600.13995160773646675No Hit
GGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAG396150.13702874296787274No Hit
TTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTC362090.1252473495929245No Hit
GTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCT329710.1140470701601346No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC320940.11101351702160564No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG307190.10625737612596448No Hit
GCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTG305510.10567626218380613No Hit
GGGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTA304730.10540645928208976No Hit
CTGCAGGCTGCCTGCACCAGGGCCCCCGCCCAGCTCCACCTGCCCCACCT301430.10426498546713588No Hit
GAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGC294620.10190939859445833No Hit
GTGGGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGG292980.10134212069854184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)