FastQCFastQC Report
Thu 16 Oct 2025
HPAP-159_exocrine_5000161_NSS0011_s_1_CAGTGCTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-159_exocrine_5000161_NSS0011_s_1_CAGTGCTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57535611
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2309470.40139836179023114No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1471980.25583807565717864No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG1282340.222877619219165No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1239440.21542136747274657No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT1174150.2040736127752254No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG1166290.2027075023153921No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC1125600.19563536050742555No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG1120060.1946724785802657No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1007780.1751576080420872No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC927290.16116801123394692No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG904890.15727477022882402No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG884140.15366830813702492No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG856810.14891820649997095No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC850910.1478927546280859No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC824980.1433859805538521No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG811160.1409839898980129No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT784600.13636771842051004No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT738620.12837614603588723No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC726140.12620705461874734No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT720810.1252806718260105No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC716280.12449333335488522No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT709590.12333057521540876No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA679200.11804862904819069No Hit
CAGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCT661810.11502615310716002No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG643320.11181249122391348No Hit
CTCGGTGCCAGCAGCTGGAGGGGCAGTGGGCAGAGAGATGGCGGACACGC583990.10150061672239824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)