FastQCFastQC Report
Thu 16 Oct 2025
HPAP-160_alpha_5000162_NSS0022_s_1_CTTACAGC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-160_alpha_5000162_NSS0022_s_1_CTTACAGC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172728183
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC5903500.3417797777679396No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT3838720.22224051300302278No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG3288910.1904095754889056No Hit
CTCAGAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCA3286920.19029436556974608No Hit
CTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATG2921280.16912584554889923No Hit
GGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCAG2470190.14301024633600182No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG2394950.1386542692920008No Hit
GGGAACGTTGCCAGCTGCCTTGTACCAGCATTACAAATAATCCAGCCACA2266150.13119746648408848No Hit
GGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTT2214180.12818869286664122No Hit
TGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACG1866230.10804432534324755No Hit
CGGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCA1795730.10396276790568683No Hit
GTGATTTTGGTCTGAATCAACCAGTTTATAAAGTCCCTGGCGGCAAGATT1748790.10124520327988398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)