FastQCFastQC Report
Thu 16 Oct 2025
HPAP-160_alpha_5000162_NSS0022_s_2_CTTACAGC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-160_alpha_5000162_NSS0022_s_2_CTTACAGC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165342495
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC5609940.3392920858004471No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT3651710.22085731801736752No Hit
CTCAGAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCA3147990.19039207071358152No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG3142130.19003765486906435No Hit
CTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATG2777750.16799976315828546No Hit
GGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCAG2330480.14094864118265543No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG2282300.1380346897511133No Hit
GGGAACGTTGCCAGCTGCCTTGTACCAGCATTACAAATAATCCAGCCACA2158490.1305465966265962No Hit
GGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTT2121820.12832877597498452No Hit
TGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACG1767670.10690959997912214No Hit
CGGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCA1702880.10299106711798439No Hit
GTGATTTTGGTCTGAATCAACCAGTTTATAAAGTCCCTGGCGGCAAGATT1661550.10049140724530617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)