FastQCFastQC Report
Thu 16 Oct 2025
HPAP-161_alpha_5000165_NSS0011_s_2_AGACGCTA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-161_alpha_5000165_NSS0011_s_2_AGACGCTA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17765690
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT841140.47346317536780164No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC303550.1708630511958725No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT282540.1590368851420913No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA209050.1176706336764854No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC200030.11259343149632804No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA185200.10424588068349723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)